14808 Xcl1 Status: ES cell colonies screened / QC positives
| Gene | Xcl1 |
| Chromosome | 1 |
| Deletion Start | 166,865,618 |
| Deletion End | 166,861,834 |
| Deletion Size | 3,785 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 166,865,597 | 166,865,618 | AGACTTCTCCTCCTGACTTTCC |
| TUP | 166,865,576 | 166,865,596 | TGGGAGTCTGCTGCCTCACCC |
| TUR | 166,865,516 | 166,865,538 | ATGAGCCCACCTCTTCTTTATCC |
| TDF | 166,861,929 | 166,861,948 | TCCACCAGCACAGCGATAAC |
| TDP | 166,861,903 | 166,861,928 | CCTGACTGGGTAACAGCCTCCAGGAC |
| TDR | 166,861,853 | 166,861,873 | GCAATGGGTTTGGGAACTGAG |
PCR Primers
| Primer | Sequence |
| SU | TTCAGGATGGAAGATCCGAC |
| SD | GGAAACTGAAATAGATCGAGCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14808A-A7 | VGB6 | C57BL/6NTac | |
| 14808A-E11 | VGB6 | C57BL/6NTac | |
| 14808A-H9 | VGB6 | C57BL/6NTac | |
| 14808A-F4 | VGB6 | C57BL/6NTac | |
| 14808A-H5 | VGB6 | C57BL/6NTac | |
| 14808A-G8 | VGB6 | C57BL/6NTac | |
