14811 Apoc2 Status: Cancelled
| Gene | Apoc2 |
| Chromosome | 7 |
| Deletion Start | 19,673,598 |
| Deletion End | 19,671,681 |
| Deletion Size | 1,918 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 19,673,443 | 19,673,465 | TCAACCATTCCTTCGTCTTCCTC |
| TUP | 19,673,417 | 19,673,439 | TGCCTCGACTCACCCAAAGTCCC |
| TUR | 19,673,344 | 19,673,362 | GGCTGCCCGAGTCATCTTC |
| TDF | 19,671,771 | 19,671,792 | ACCAGCTCCTTACTCTCCTGAG |
| TDP | 19,671,742 | 19,671,765 | AGTAGCTGCCAGGCAAGCCCCAAG |
| TDR | 19,671,704 | 19,671,722 | AGGCGCGGTGTCTGTCATC |
PCR Primers
| Primer | Sequence |
| SU | AAAAGCAACCCTCTAGAGTAG |
| SD | AGTGAAAGCTACAGAATGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 122 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
