14822 Btd Status: Cancelled
| Gene | Btd |
| Chromosome | 14 |
| Deletion Start | 31,641,128 |
| Deletion End | 31,667,971 |
| Deletion Size | 26,844 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,642,012 | 31,642,035 | TTGCCTGTAGAGATTAAGCAGAAG |
| TUP | 31,642,036 | 31,642,061 | TGACGTGACTGTCTTCCAAGTGCAAA |
| TUR | 31,642,062 | 31,642,080 | TGGATGGAACAGCAGTGAC |
| TDF | 31,667,543 | 31,667,562 | GCCTCTGTTGCCACTTACTG |
| TDP | 31,667,563 | 31,667,585 | TATGAGAGGCCCACCCTGTCCAA |
| TDR | 31,667,607 | 31,667,626 | CGGTGTGGAGTCCGTCAAAG |
PCR Primers
| Primer | Sequence |
| SU | CTGCGGAGTTACTTTCTGGC |
| SD | ACAGAAAGTGATCGGGCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
