14828 Col16a1 Status: ES cell colonies screened / QC no positives
| Gene | Col16a1 |
| Chromosome | 4 |
| Deletion Start | 130,049,670 |
| Deletion End | 130,079,529 |
| Deletion Size | 29,860 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 130,050,139 | 130,050,158 | TTCCCTCTCATGGTCAGGAC |
| TUP | 130,050,161 | 130,050,184 | CATCCTCCAGGCATAGGCTCCTCC |
| TUR | 130,050,196 | 130,050,216 | GGGCAGTCATCAGAGATCCAG |
| TDF | 130,079,128 | 130,079,148 | CCTGGGTCCTTCTGTCCTTTC |
| TDP | 130,079,156 | 130,079,181 | CCCATCAGAGCATCTGTGGTGACTGT |
| TDR | 130,079,191 | 130,079,209 | AGGCTGGATGAGCCGTCTG |
PCR Primers
| Primer | Sequence |
| SU | CCTTGCTTTCTCTGCCTCTG |
| SD | CAGGAGAGGCAGAGAAGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
