14835 Rln3 Status: Cancelled
| Gene | Rln3 |
| Chromosome | 8 |
| Deletion Start | 84,044,953 |
| Deletion End | 84,043,028 |
| Deletion Size | 1,926 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,044,188 | 84,044,207 | CAGGAGGCCAATCAGGAGAG |
| TUP | 84,044,165 | 84,044,187 | TGCAGCTCCTTAGAGGCGAGACA |
| TUR | 84,044,101 | 84,044,121 | CCTCAGCTTCCCAGAGTGTTG |
| TDF | 84,043,957 | 84,043,981 | GTGGTGAATGATGGACAGACAGATG |
| TDP | 84,043,923 | 84,043,946 | CAGGTTAGGAGTGTGCCTGCCTCA |
| TDR | 84,043,890 | 84,043,913 | GGACACAGCCATTTAGCCATAGAG |
PCR Primers
| Primer | Sequence |
| SU | AAGAAAGGAGAGGTGGTGGC |
| SD | GAGGTTAGCCAGGGACAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
