| Gene | Klk6 |
| Chromosome | 7 |
| Deletion Start | 43,826,081 |
| Deletion End | 43,831,699 |
| Deletion Size | 5,619 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,826,802 | 43,826,821 | GGTCGGAGGAACAGGAGAAG |
| TUP | 43,826,826 | 43,826,852 | TTCATGGAGGCCCGTGTTTGAAGGACT |
| TUR | 43,826,859 | 43,826,876 | AGAGGGCAGCCTGGAAAG |
| TDF | 43,831,554 | 43,831,575 | GCCCTGTGGATCAAAGGAGAAG |
| TDP | 43,831,577 | 43,831,604 | CAGGAGTTTACACCGATGTCTGCACTCA |
| TDR | 43,831,635 | 43,831,656 | TGTCTCATGTCACAGCCACTTG |
PCR Primers
| Primer | Sequence |
| SU | CCCACACTGTCTTGCATTTG |
| SD | CTGAGCATTTCTCCAGCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14841A-A11 | VGB6 | C57BL/6NTac | |
| 14841A-B10 | VGB6 | C57BL/6NTac | |
| 14841A-F9 | VGB6 | C57BL/6NTac | |
| 14841A-E4 | VGB6 | C57BL/6NTac | |
| 14841A-D11 | VGB6 | C57BL/6NTac | |
| 14841A-A2 | VGB6 | C57BL/6NTac | |
