14845 Npvf Status: Cancelled
| Gene | Npvf |
| Chromosome | 6 |
| Deletion Start | 50,654,390 |
| Deletion End | 50,650,869 |
| Deletion Size | 3,522 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 50,653,521 | 50,653,541 | TCCAGTGTTTAGGGTGGAACC |
| TUP | 50,653,487 | 50,653,511 | TATTCACTGCACGTTACGTGGCTCC |
| TUR | 50,653,455 | 50,653,478 | GAAGAGCCAAAGTCCAACAATCTG |
| TDF | 50,651,056 | 50,651,075 | GGCTTGGCTCAGACTCACAG |
| TDP | 50,651,032 | 50,651,055 | AGATCCACCTGCCTCTGTCTCCCA |
| TDR | 50,650,987 | 50,651,008 | ACAGCCCATGCGTAATGTAGAC |
PCR Primers
| Primer | Sequence |
| SU | AACATCCTGTTTGTTTAGAATC |
| SD | AGAGACTTCTTTATTTACAATACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
