| Gene | Gif |
| Chromosome | 19 |
| Deletion Start | 11,747,650 |
| Deletion End | 11,763,322 |
| Deletion Size | 15,673 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 11,748,549 | 11,748,567 | AACGTGGAGGCCCAGAAAC |
| TUP | 11,748,568 | 11,748,591 | TCCTGACTTACCAGCTCATGGCCA |
| TUR | 11,748,628 | 11,748,649 | TGTGCGTGGAAGATTCAGGTTG |
| TDF | 11,762,645 | 11,762,664 | AACCCAGCACCCACATCAAG |
| TDP | 11,762,665 | 11,762,689 | TGCCTCACAACAGCCTGTAATCCCA |
| TDR | 11,762,690 | 11,762,710 | GTGGAGGACAGAAGAAGGAAC |
PCR Primers
| Primer | Sequence |
| SU | AAGCATCAGGAGGCTATTGG |
| SD | CTGAGCTAGAAACCATCCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14849A-B3 | VGB6 | C57BL/6NTac | |
| 14849A-C8 | VGB6 | C57BL/6NTac | |
| 14849A-F4 | VGB6 | C57BL/6NTac | |
| 14849A-C9 | VGB6 | C57BL/6NTac | |
