14851 Ccl22 Status: ES cell colonies screened / QC positives
| Gene | Ccl22 |
| Chromosome | 8 |
| Deletion Start | 97,269,617 |
| Deletion End | 97,273,875 |
| Deletion Size | 4,259 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 97,269,704 | 97,269,724 | GAGGGTGGAGACCAGAGAATC |
| TUP | 97,269,725 | 97,269,751 | TGGAAAGAGTCCAAGAGTGAGTCTGCC |
| TUR | 97,269,768 | 97,269,791 | GGAAGTGCCAGAGTTAGTCCAAAG |
| TDF | 97,273,454 | 97,273,475 | GCCCTTAGTAGTGTCTGCTTTC |
| TDP | 97,273,477 | 97,273,504 | TGAGCAGATCGGTATGTATCTAGACGGC |
| TDR | 97,273,583 | 97,273,603 | GCTCCTTGTTAGCAAGTCAGC |
PCR Primers
| Primer | Sequence |
| SU | TGTCCTTATCCATGTGCCAG |
| SD | ATGCCCACTGGGACTGATAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 150 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14851B-G2 | VGB6 | C57BL/6NTac | |
| 14851B-D3 | VGB6 | C57BL/6NTac | |
