14855 Nenf Status: Cancelled
| Gene | Nenf |
| Chromosome | 1 |
| Deletion Start | 191,318,089 |
| Deletion End | 191,306,912 |
| Deletion Size | 11,178 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 191,317,632 | 191,317,654 | TGATCCAAGCCTTTCCTTTCCTG |
| TUP | 191,317,609 | 191,317,628 | CGGTGTCCCGGGCCTACTGA |
| TUR | 191,317,569 | 191,317,590 | CCACCAGCCCAGACACTAAATG |
| TDF | 191,307,344 | 191,307,364 | AAGGCAGTTTCCCAGGGTGAG |
| TDP | 191,307,323 | 191,307,343 | TCTCCACGTGTGCTGACCGCT |
| TDR | 191,307,290 | 191,307,312 | TGGGACCAAGTTTCTTGCATTTG |
PCR Primers
| Primer | Sequence |
| SU | AGAGAAAGGAGGAGAGGACA |
| SD | TTACCTCCTGGCCTCATCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
