| Gene | Cpb1 |
| Chromosome | 3 |
| Deletion Start | 20,275,647 |
| Deletion End | 20,249,592 |
| Deletion Size | 25,079 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,275,187 | 20,275,207 | GCTGACTGTGACGGCTAAATG |
| TUP | 20,275,163 | 20,275,183 | TGGCTCTGTCTTGAAGGTCAT |
| TUR | 20,275,125 | 20,275,144 | TGTCTTGGAAGGGAAGTGAC |
| TDF | 20,249,732 | 20,249,753 | CTTTGGCTTTCTGCTTCCTGAG |
| TDP | 20,249,706 | 20,249,730 | CTCAGATCCGCCAAACCTGTGAGGA |
| TDR | 20,249,684 | 20,249,705 | GTACTTGACTGCAAGCATTGTC |
PCR Primers
| Primer | Sequence |
| SU | GTCGATCTCAAATCAAAATGAG |
| SD | AAACCCAAGCCAGGATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14858A-B5 | VGB6 | C57BL/6NTac | |
| 14858B-A8 | VGB6 | C57BL/6NTac | |
| 14858B-B5 | VGB6 | C57BL/6NTac | |
| 14858B-E9 | VGB6 | C57BL/6NTac | |
| 14858B-G9 | VGB6 | C57BL/6NTac | |
| 14858C-A7 | VGB6 | C57BL/6NTac | |
