Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

14869 St6galnac5 Status: ES cell colonies screened / QC positives

Gene	St6galnac5
Chromosome	3
Deletion Start	152,982,014
Deletion End	152,981,063
Deletion Size	952
Genome Build	38

MGI ID	MGI:1349471
NCBI Gene ID	26938
BAC ID	371C17
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 3

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	152,981,908	152,981,929	GAAGCCTCTCCGGGTATCTATC
TUP	152,981,884	152,981,907	TCTGTGCCAAGAGGTCACCTGTCA
TUR	152,981,850	152,981,869	CCGGATTCCAAGCCAGGTAG
TDF	152,981,491	152,981,509	ACCTGCCCTCCACTGTGAG
TDP	152,981,459	152,981,483	CTGTGAGCAGCGATGCAGGAGCATC
TDR	152,981,427	152,981,446	CAGAGGTGACACGGTCTTGC

PCR Primers

Primer	Sequence
SU	AAATGGCTACCGTGTTCAGG
SD	ACTGAACATCCATCTGCAGC
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	80 bp	+	+	-
TDF	TDR	83 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
14869A-H8	VGB6	C57BL/6NTac
14869A-G2	VGB6	C57BL/6NTac
14869A-G5	VGB6	C57BL/6NTac
14869A-C5	VGB6	C57BL/6NTac