14873 Tor3a Status: ES cell colonies screened / QC no positives
| Gene | Tor3a |
| Chromosome | 1 |
| Deletion Start | 156,674,302 |
| Deletion End | 156,655,665 |
| Deletion Size | 18,638 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 156,674,036 | 156,674,055 | CTCACCTGCCAGTCTCACTC |
| TUP | 156,674,013 | 156,674,034 | TAAGCACTGCGTACTGCAGGCC |
| TUR | 156,673,928 | 156,673,950 | CAGAGCAGCCAGACCTTTAGTTG |
| TDF | 156,656,506 | 156,656,523 | ACGACGCAGCACTTGGAG |
| TDP | 156,656,477 | 156,656,501 | CCCTTCAGGCTGAGATCATGGAGGC |
| TDR | 156,656,434 | 156,656,455 | TCTTGGCAAGGCTGTATGTATC |
PCR Primers
| Primer | Sequence |
| SU | AGAATAGCCTGCATCCCGAG |
| SD | GCTGGTCTCACATGCAGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 128 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
