| Gene | Bpifb2 |
| Chromosome | 2 |
| Deletion Start | 153,875,909 |
| Deletion End | 153,895,099 |
| Deletion Size | 19,191 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 153,875,970 | 153,875,990 | CTGTCTCCTTGCCTGTCATTG |
| TUP | 153,875,991 | 153,876,014 | TGGTCCGACTCAACAAGGCAGCAT |
| TUR | 153,876,059 | 153,876,079 | TCATTCAAACCTGGCTCTCAC |
| TDF | 153,894,984 | 153,895,005 | TCCAGGGCTATGTTGTGGTATC |
| TDP | 153,895,009 | 153,895,032 | TGGACTTGCCTACCAGCACTGAGC |
| TDR | 153,895,038 | 153,895,057 | TGAGGAACTGGCTCTTGTGG |
PCR Primers
| Primer | Sequence |
| SU | TCTGTCTGCTGTGCCAATTC |
| SD | GGAGGTGAGAAGTGACAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14877A-C10 | VGB6 | C57BL/6NTac | |
| 14877B-C12 | VGB6 | C57BL/6NTac | |
| 14877C-B10 | VGB6 | C57BL/6NTac | |
