14879 Mxra7 Status: ES cell colonies screened / QC no positives
| Gene | Mxra7 |
| Chromosome | 11 |
| Deletion Start | 116,827,995 |
| Deletion End | 116,804,504 |
| Deletion Size | 23,492 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,827,356 | 116,827,377 | GCAAGAAGAGAAGTTGCCTGTC |
| TUP | 116,827,322 | 116,827,351 | AGGTCAACTCGAATCCAGATTCCAGAGTCA |
| TUR | 116,827,283 | 116,827,302 | CCTCAGTCCCGTCCTTCCAG |
| TDF | 116,805,199 | 116,805,216 | TGCGCTGGTCAGATGGTG |
| TDP | 116,805,174 | 116,805,198 | TGGCCAGAGTCTCACAAACCACAAA |
| TDR | 116,805,117 | 116,805,140 | TCAAAGCAAGTGTGGATGCAGATG |
PCR Primers
| Primer | Sequence |
| SU | TTGCACTACCATCTCTAAATG |
| SD | GCTTGCTTCAACTACCACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
