| Gene | Lyzl6 |
| Chromosome | 11 |
| Deletion Start | 103,636,893 |
| Deletion End | 103,631,265 |
| Deletion Size | 5,629 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 103,636,532 | 103,636,553 | TTCCCTCACAGAGCTTACATTC |
| TUP | 103,636,509 | 103,636,531 | TAGCACAGCAGCGAGCACTCACG |
| TUR | 103,636,455 | 103,636,474 | GCCTGCACCACATTCTTCAC |
| TDF | 103,631,355 | 103,631,375 | CACTGTTTAGGACGACCACTC |
| TDP | 103,631,330 | 103,631,354 | TCCTACTGGATGACGGGATGCCATC |
| TDR | 103,631,308 | 103,631,326 | GTCTGCACCCTGGCTCATC |
PCR Primers
| Primer | Sequence |
| SU | TGAGCAATGGGTATATGGAGG |
| SD | TGCCCACTGCAATATTTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14882A-H11 | VGB6 | C57BL/6NTac | |
| 14882A-H9 | VGB6 | C57BL/6NTac | |
