14890 Crispld1 Status: ES cell colonies screened / QC no positives
| Gene | Crispld1 |
| Chromosome | 1 |
| Deletion Start | 17,728,660 |
| Deletion End | 17,764,216 |
| Deletion Size | 35,557 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,728,672 | 17,728,691 | CAGGAGTGGCTCAGAGTGAC |
| TUP | 17,728,692 | 17,728,716 | CGCACTGCTATTTGTGGCTAGAGCA |
| TUR | 17,728,732 | 17,728,753 | CCAGCAAAGTGGCATTAGGAAC |
| TDF | 17,764,072 | 17,764,093 | CCCTACAGGAGGAAAGGCATTC |
| TDP | 17,764,100 | 17,764,124 | TTCGCTGTTGTTTGACCGGGAACAC |
| TDR | 17,764,137 | 17,764,157 | GCATTTGGAGCAGGCTTTCTG |
PCR Primers
| Primer | Sequence |
| SU | ACCAGAGATGAAATGCTGGG |
| SD | CCCAGTCCTAAGGGTAAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
