14898 B3gnt7 Status: ES cell colonies screened / QC no positives
| Gene | B3gnt7 |
| Chromosome | 1 |
| Deletion Start | 86,303,547 |
| Deletion End | 86,306,238 |
| Deletion Size | 2,692 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 86,304,138 | 86,304,160 | GGGTAGGAGGACTTAACATTCAC |
| TUP | 86,304,166 | 86,304,186 | CTGCGCCTCCGCAGGCTTAAA |
| TUR | 86,304,199 | 86,304,218 | GCCCACGAAGCACGTAGTAG |
| TDF | 86,305,568 | 86,305,589 | CAAGAGGAGCGAACCCACTATC |
| TDP | 86,305,592 | 86,305,613 | CAGCTGCTGGCCTACGAGGACC |
| TDR | 86,305,634 | 86,305,654 | GCTGTCAAGGAAATCCCACTG |
PCR Primers
| Primer | Sequence |
| SU | TCTGAGATTCTGGCTCTTCC |
| SD | TCCCTGTGAGGACAAGAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
