| Gene | Mmp21 |
| Chromosome | 7 |
| Deletion Start | 133,680,043 |
| Deletion End | 133,674,373 |
| Deletion Size | 5,671 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 133,679,522 | 133,679,541 | CCAGTCATGGCTCTGAAGTC |
| TUP | 133,679,493 | 133,679,521 | TGGGTCTTACCTCAAGCAGACAGATGACC |
| TUR | 133,679,443 | 133,679,465 | GCACCCTGGAACCAGAAGTTTAG |
| TDF | 133,674,789 | 133,674,811 | GACAATGGCAGACAGATGGAAAC |
| TDP | 133,674,755 | 133,674,784 | AGAACTTGACCTCTACAGAACTCAGTGCGA |
| TDR | 133,674,721 | 133,674,744 | GATAGATTTGCTGAGGCTTGTTGG |
PCR Primers
| Primer | Sequence |
| SU | CAACACAGGAGAGCAATCCAC |
| SD | TGTGCCCTTTCCTAGACCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14900B-A4 | VGB6 | C57BL/6NTac | |
| 14900B-D11 | VGB6 | C57BL/6NTac | |
| 14900B-E1 | VGB6 | C57BL/6NTac | |
| 14900B-G7 | VGB6 | C57BL/6NTac | |
| 14900B-H5 | VGB6 | C57BL/6NTac | |
