14903 Ccdc134 Status: ES cell colonies screened / QC positives
| Gene | Ccdc134 |
| Chromosome | 15 |
| Deletion Start | 81,960,501 |
| Deletion End | 81,971,463 |
| Deletion Size | 10,963 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,961,260 | 81,961,280 | GCCCAGTCTCACACCACTAAC |
| TUP | 81,961,283 | 81,961,306 | AGCCACACCTCCTAACCCTGTCGC |
| TUR | 81,961,339 | 81,961,360 | CAGCATGGGAAGATGGGTAGAG |
| TDF | 81,970,764 | 81,970,784 | GCAAAGGGTAGCTGGAGAAAG |
| TDP | 81,970,786 | 81,970,810 | TTTGAGGCTGAAGTCACCCACATCC |
| TDR | 81,970,817 | 81,970,837 | TGATGCTGGCAAGAAGAGATG |
PCR Primers
| Primer | Sequence |
| SU | ACATTGTTCAGACCTGTCGC |
| SD | GTTCTGCAGAGAGCTGGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14903A-A2 | VGB6 | C57BL/6NTac | |
| 14903A-A3 | VGB6 | C57BL/6NTac | |
| 14903A-G1 | VGB6 | C57BL/6NTac | |
| 14903A-G10 | VGB6 | C57BL/6NTac | |
| 14903A-D10 | VGB6 | C57BL/6NTac | |
