| Gene | Ager |
| Chromosome | 17 |
| Deletion Start | 34,597,897 |
| Deletion End | 34,600,875 |
| Deletion Size | 2,979 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,598,110 | 34,598,129 | TCCACCCTCCCACAATGATG |
| TUP | 34,598,130 | 34,598,156 | CTATCACCCAGGAGCTGTAGCTGGTGG |
| TUR | 34,598,158 | 34,598,176 | TCCGGGCTGTGATGTTCTG |
| TDF | 34,600,715 | 34,600,734 | CGGAAAGCCAGGAGGATGAG |
| TDP | 34,600,741 | 34,600,766 | CGTGCAGAGCTGAATCAGTCAGAGGA |
| TDR | 34,600,771 | 34,600,791 | GGCACCATTCTCTGGCATCTC |
PCR Primers
| Primer | Sequence |
| SU | AACTTGCCCACTAGCAAACC |
| SD | TGGTGTTTAAAGGTGGGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14925A-C7 | VGB6 | C57BL/6NTac | |
| 14925A-C10 | VGB6 | C57BL/6NTac | |
| 14925A-D5 | VGB6 | C57BL/6NTac | |
| 14925A-F1 | VGB6 | C57BL/6NTac | |
| 14925A-H8 | VGB6 | C57BL/6NTac | |
| 14925A-H1 | VGB6 | C57BL/6NTac | |
