14926 Prox1 Status: ES cell colonies screened / QC no positives
| Gene | Prox1 |
| Chromosome | 1 |
| Deletion Start | 190,162,243 |
| Deletion End | 190,123,123 |
| Deletion Size | 39,121 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 190,161,323 | 190,161,341 | GGACCCAGGGCAGTTCATC |
| TUP | 190,161,294 | 190,161,315 | CCCGAGCCCTAATCAGGGAGCA |
| TUR | 190,161,269 | 190,161,292 | CTTAGGCTTGTTCTCAGCCATCTC |
| TDF | 190,123,295 | 190,123,317 | GGAAGAAGGCCATTTACAAGGTC |
| TDP | 190,123,264 | 190,123,291 | TGCAAGCTGGATAGTGAAGTTCCTGAGA |
| TDR | 190,123,212 | 190,123,234 | TGCAATCTCTACTCGTGAAGGAG |
PCR Primers
| Primer | Sequence |
| SU | AATCAATTGCTCATCAGAGGC |
| SD | TCAGGCTCAAATCAAACAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
