14940 Atg10 Status: ES cell colonies screened / QC no positives
| Gene | Atg10 |
| Chromosome | 13 |
| Deletion Start | 91,208,419 |
| Deletion End | 91,154,222 |
| Deletion Size | 54,198 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 91,208,331 | 91,208,348 | AGGCGATGGCTGGGAATG |
| TUP | 91,208,300 | 91,208,325 | CAGCCAAGGTGAGAGCAGAAGATGCA |
| TUR | 91,208,254 | 91,208,274 | AGCTCTGCTGTGCAGTTAGAC |
| TDF | 91,155,178 | 91,155,196 | GCTCTGGTTGGGAGGACTG |
| TDP | 91,155,147 | 91,155,172 | TGATCCTAGCCTTCATCAGGCAGCCT |
| TDR | 91,155,107 | 91,155,128 | CACGTTGGGTTCCTTGGATCTG |
PCR Primers
| Primer | Sequence |
| SU | ACAGCCCAGAGTGTTTGGTC |
| SD | ACTAGAACTGAAGGATCACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
