14941 Bcap31 Status: ES cell colonies screened / QC no positives
| Gene | Bcap31 |
| Chromosome | X |
| Deletion Start | 73,714,881 |
| Deletion End | 73,686,523 |
| Deletion Size | 28,359 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,714,417 | 73,714,437 | CATTGTTGCCGATTTGCTGAC |
| TUP | 73,714,387 | 73,714,413 | TGACCTCCTAGCTAACTCCTACTGGCA |
| TUR | 73,714,339 | 73,714,361 | GCTTCCATCCTTCATAGGCTGAG |
| TDF | 73,686,816 | 73,686,838 | GGATTGAACCCAAGTCCTCATAC |
| TDP | 73,686,786 | 73,686,814 | TTTATGGCAGATGCTATCTCCCAACTCCC |
| TDR | 73,686,746 | 73,686,769 | GGCCCTTAAACAATTACCTTCCAG |
PCR Primers
| Primer | Sequence |
| SU | CCTTACAAGGGAGCCTGATG |
| SD | GAGGAGCAGCAGAACCATTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
