14951 Ghitm Status: ES cell colonies screened / QC no positives
| Gene | Ghitm |
| Chromosome | 14 |
| Deletion Start | 37,133,761 |
| Deletion End | 37,122,127 |
| Deletion Size | 11,635 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,133,697 | 37,133,717 | TTTCCAGCCCACTTTCATCAC |
| TUP | 37,133,668 | 37,133,696 | CAAGGCCTCTCCACTTGTGAAGAATTCCA |
| TUR | 37,133,631 | 37,133,650 | ACCCTGCTGGGTGTTACGAG |
| TDF | 37,122,859 | 37,122,879 | CGTGTCCGACCTGTTGGAATG |
| TDP | 37,122,819 | 37,122,846 | TGGAATGTGTAAGGAAGGAAAACAGCCC |
| TDR | 37,122,795 | 37,122,814 | CACGCACAAGGAGAAGTGTC |
PCR Primers
| Primer | Sequence |
| SU | TGGAGTAAATATCAGGCCAAGC |
| SD | CTGCAGGCTTCCACAATATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
