14953 Il17d Status: Cancelled
| Gene | Il17d |
| Chromosome | 14 |
| Deletion Start | 57,524,910 |
| Deletion End | 57,542,671 |
| Deletion Size | 17,762 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,525,743 | 57,525,762 | CTGGAGCGGTATAGGTGGAC |
| TUP | 57,525,765 | 57,525,784 | TGCTTGAAGGAAGGGGTCGT |
| TUR | 57,525,850 | 57,525,869 | CTCGGGCACTACACCATCTC |
| TDF | 57,542,190 | 57,542,211 | CCAGAGTGCTTACTGCGGATTG |
| TDP | 57,542,214 | 57,542,238 | TTGTCAGATCCAGCCTTCTCAGGCC |
| TDR | 57,542,282 | 57,542,302 | CAGGAGCATCACTGCGAGTTC |
PCR Primers
| Primer | Sequence |
| SU | ATGAAATCCCAGAAGAGGAAG |
| SD | GACCTTAGGTGCCCAGATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 127 bp | + | + | - |
| TDF | TDR | 113 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
