| Gene | Pam16 |
| Chromosome | 16 |
| Deletion Start | 4,624,850 |
| Deletion End | 4,616,448 |
| Deletion Size | 8,403 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,624,532 | 4,624,552 | CGGCGATATTGGTGTTGTGTC |
| TUP | 4,624,498 | 4,624,523 | TTTAACCGCGTTTGACAGTTGGCTCA |
| TUR | 4,624,460 | 4,624,482 | TCAATTCTAGCAAGTCTCAGGTC |
| TDF | 4,617,000 | 4,617,023 | CCCAGGCAGAACAACTAAAGAAAC |
| TDP | 4,616,977 | 4,616,999 | ATGGCAGCCACCTGTCCCTCTGA |
| TDR | 4,616,950 | 4,616,967 | GCAGGAAGCTGGTGTGAC |
PCR Primers
| Primer | Sequence |
| SU | ACCAAACTGCACCAAGTCTG |
| SD | GAAGGCCTCCTATGTGGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14958A-E3 | VGB6 | C57BL/6NTac | |
| 14958A-G10 | VGB6 | C57BL/6NTac | |
| 14958A-C11 | VGB6 | C57BL/6NTac | |
| 14958A-D3 | VGB6 | C57BL/6NTac | |
| 14958A-E9 | VGB6 | C57BL/6NTac | |
| 14958A-E8 | VGB6 | C57BL/6NTac | |
