14959 Mcfd2 Status: ES cell colonies screened / QC no positives
| Gene | Mcfd2 |
| Chromosome | 17 |
| Deletion Start | 87,258,070 |
| Deletion End | 87,255,830 |
| Deletion Size | 2,241 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,257,253 | 87,257,271 | AGCCAGAGGCGGAGATGTC |
| TUP | 87,257,228 | 87,257,252 | CCCACAGGAACTGCAGCTCCATTAC |
| TUR | 87,257,183 | 87,257,201 | TAGGCCGTCAAGCAAACTG |
| TDF | 87,255,977 | 87,255,997 | GTCCTGAGGGACGATGACAAG |
| TDP | 87,255,951 | 87,255,976 | AACAACGACGGCTACATCGACTACGC |
| TDR | 87,255,910 | 87,255,931 | GGAAAGAGCCAACGGTCTACTG |
PCR Primers
| Primer | Sequence |
| SU | CGTTCTCAGTGCTCTTGTCTG |
| SD | TGGCTTTCACACTCTCCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
