14961 Ms4a13 Status: ES cell colonies screened / QC no positives
| Gene | Ms4a13 |
| Chromosome | 19 |
| Deletion Start | 11,192,975 |
| Deletion End | 11,169,904 |
| Deletion Size | 23,072 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 11,192,741 | 11,192,760 | TGGGCTGGTTAAGCTGTATC |
| TUP | 11,192,715 | 11,192,740 | TGCATTCAGGAATCAGAGGGCAAACA |
| TUR | 11,192,679 | 11,192,698 | CGGGCATTGAGGCTTTGTAG |
| TDF | 11,170,714 | 11,170,734 | GCGATCTGCGTCTTGAAGGTG |
| TDP | 11,170,684 | 11,170,712 | TGCCAGACAGGTGGTGATGTTTATGTTCC |
| TDR | 11,170,656 | 11,170,676 | GCATTCTGGGAGAGTCTGAAC |
PCR Primers
| Primer | Sequence |
| SU | TTCCTTATCATCCAGAAACACTG |
| SD | CGCTTTGTGGAGGTAGAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
