14969 Slc12a9 Status: ES cell colonies screened / QC no positives
| Gene | Slc12a9 |
| Chromosome | 5 |
| Deletion Start | 137,332,633 |
| Deletion End | 137,314,941 |
| Deletion Size | 17,693 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 137,332,250 | 137,332,267 | TGTGCAGGGTTCGTGGTG |
| TUP | 137,332,224 | 137,332,245 | ACGCTGGGCTGTTGCAGGCCTT |
| TUR | 137,332,180 | 137,332,202 | GGACTGTTAGTGCCAGAATGATG |
| TDF | 137,315,083 | 137,315,102 | CCACAGCCCTTACCTTCCTG |
| TDP | 137,315,058 | 137,315,079 | CTGCCTCGACCGCCTGCTGATC |
| TDR | 137,314,993 | 137,315,012 | AAGGGTGGGACCCAGATCAC |
PCR Primers
| Primer | Sequence |
| SU | TGGGAGGAGGTGTTTACTGG |
| SD | CCACCAGCCCATAGTGTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
