14988 Slc25a15 Status: ES cell colonies screened / QC no positives
| Gene | Slc25a15 |
| Chromosome | 8 |
| Deletion Start | 22,395,764 |
| Deletion End | 22,377,839 |
| Deletion Size | 17,926 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 22,395,114 | 22,395,133 | GGTCAGCAAGCACTCTTCAC |
| TUP | 22,395,090 | 22,395,113 | TGCTGAGCCATTTACCCAGCTTCC |
| TUR | 22,395,045 | 22,395,065 | CCCAATGCCTGCAACAGTAAC |
| TDF | 22,378,273 | 22,378,292 | TCGGGTTCTCTTGCTAGTCG |
| TDP | 22,378,251 | 22,378,271 | TTGGCTCCTCAGTGGCCTCCA |
| TDR | 22,378,209 | 22,378,230 | TGCAGTATAGACATGGCAAAGC |
PCR Primers
| Primer | Sequence |
| SU | AGGCCTCTGAAACTGTCTGC |
| SD | GGCCATCATAATTGCATGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
