14991 Slc25a24 Status: ES cell colonies screened / QC no positives
| Gene | Slc25a24 |
| Chromosome | 3 |
| Deletion Start | 109,123,208 |
| Deletion End | 109,149,565 |
| Deletion Size | 26,358 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 109,123,810 | 109,123,831 | CGCATTGATCTGAAGGGTTGAC |
| TUP | 109,123,836 | 109,123,864 | TGCAAAGCAATTGGACTTTCTCTGAGTCA |
| TUR | 109,123,881 | 109,123,899 | GCCAAGGAGCCTGGATGTC |
| TDF | 109,148,632 | 109,148,651 | GTGCCTCCCTCATCCTCTTC |
| TDP | 109,148,655 | 109,148,677 | TCCTCCAGCTTTCCCTGCTCCTG |
| TDR | 109,148,699 | 109,148,721 | TCTTCCCTCAAGTGGCTAACATC |
PCR Primers
| Primer | Sequence |
| SU | TTCTGGAGTCAGGAAGCTGG |
| SD | TGTTTGAAGTAACACTGAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
