15003 Slc35b3 Status: ES cell colonies screened / QC no positives
| Gene | Slc35b3 |
| Chromosome | 13 |
| Deletion Start | 38,955,846 |
| Deletion End | 38,932,742 |
| Deletion Size | 23,105 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,955,280 | 38,955,304 | TGTGTAAGTGTATCACATTCGTGTC |
| TUP | 38,955,257 | 38,955,279 | TGTTGCCCTAGGAACCCAGAGGG |
| TUR | 38,955,219 | 38,955,240 | CCTCCTTTCGTCCCAATTCCAG |
| TDF | 38,933,472 | 38,933,491 | CCCTGGGCATCCATCAACTG |
| TDP | 38,933,446 | 38,933,471 | CCAGTACCTTCGCTAGGTGAGGCTTC |
| TDR | 38,933,386 | 38,933,408 | GGTTCCCTCTGATAAGCAGACTC |
PCR Primers
| Primer | Sequence |
| SU | AGGCATGCGACCACATATAG |
| SD | GGAGACACAGTGCCACTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
