| Gene | Slc35c2 |
| Chromosome | 2 |
| Deletion Start | 165,283,365 |
| Deletion End | 165,277,122 |
| Deletion Size | 6,244 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 165,283,294 | 165,283,315 | GGGCCTGGTCCTTCTATACTAC |
| TUP | 165,283,266 | 165,283,293 | TGCTTCTCCATAGGCATCACGTTCTACA |
| TUR | 165,283,228 | 165,283,248 | GCACTGGCAAGGTCTGGTTAC |
| TDF | 165,277,576 | 165,277,595 | GCCTTTACCCACAGGAAGTC |
| TDP | 165,277,555 | 165,277,575 | TGCACCCTGCTGTTGGCAGCT |
| TDR | 165,277,510 | 165,277,530 | AAAGCCCAGCCAGTTCAGGAG |
PCR Primers
| Primer | Sequence |
| SU | ACCCTGCCTCTCTTCTCTCC |
| SD | AGCTGCAGTCCAGGTGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15005A-H9 | VGB6 | C57BL/6NTac | |
| 15005A-E2 | VGB6 | C57BL/6NTac | |
