15013 Slc38a2 Status: ES cell colonies screened / QC no positives
| Gene | Slc38a2 |
| Chromosome | 15 |
| Deletion Start | 96,698,727 |
| Deletion End | 96,690,068 |
| Deletion Size | 8,660 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 96,698,043 | 96,698,066 | ATGTTTGGTTATCGTTTCCCTCTG |
| TUP | 96,698,014 | 96,698,042 | TGTAGCATCCAGGTACTACTTCCTTTGGA |
| TUR | 96,697,979 | 96,697,998 | CCCACAATCGCATTGCTCAG |
| TDF | 96,690,612 | 96,690,633 | CAAGGTTACCTCCTTCCGTCAG |
| TDP | 96,690,576 | 96,690,605 | TAGTGATGTGGGTAAGGAACTACAGATGCA |
| TDR | 96,690,541 | 96,690,562 | TGGGCGTCATCTTTCTTCAGTG |
PCR Primers
| Primer | Sequence |
| SU | TATTGCATCAGTTCCTCCCG |
| SD | CATCGAATGGTTCCGTTAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
