| Gene | Slc41a3 |
| Chromosome | 6 |
| Deletion Start | 90,611,912 |
| Deletion End | 90,645,874 |
| Deletion Size | 33,963 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,612,310 | 90,612,327 | TGGGCTCATGCCCTACAG |
| TUP | 90,612,330 | 90,612,355 | AGACCCGGATTCACTGGATCACTTCC |
| TUR | 90,612,366 | 90,612,388 | TGCTCCTTACTCCCTCATAGAAC |
| TDF | 90,645,525 | 90,645,545 | TCTCTGGGCCTCTGATTTGTG |
| TDP | 90,645,547 | 90,645,571 | TTTCCCTCTGCTCTTCTGGCAGGTG |
| TDR | 90,645,572 | 90,645,591 | GCCAGGTACAGCAGGATGAC |
PCR Primers
| Primer | Sequence |
| SU | GCCTGTAACAGTCAGTCCCG |
| SD | GTGAAGGTGCATGGTCACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15022A-H9 | VGB6 | C57BL/6NTac | |
| 15022A-E7 | VGB6 | C57BL/6NTac | |
| 15022A-H7 | VGB6 | C57BL/6NTac | |
