| Gene | Slc43a1 |
| Chromosome | 2 |
| Deletion Start | 84,840,777 |
| Deletion End | 84,863,107 |
| Deletion Size | 22,331 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,841,100 | 84,841,120 | GGCAGAGACAAAGCGACAAAG |
| TUP | 84,841,121 | 84,841,141 | CAGGGCTGCCCAAGACCGGAA |
| TUR | 84,841,190 | 84,841,208 | GACAGAGTGGCACCTGAAG |
| TDF | 84,862,374 | 84,862,392 | TGCTCAGTCTTTGCCACAG |
| TDP | 84,862,394 | 84,862,422 | CGACCTGTGAAAGAGGAACTCAGAGTTGA |
| TDR | 84,862,446 | 84,862,465 | GCCTGCTCCCTCCATGATTC |
PCR Primers
| Primer | Sequence |
| SU | GATCTGGTGCCTAGAGGCTG |
| SD | ATCCTGGATACAGCCAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15023A-B8 | VGB6 | C57BL/6NTac | |
| 15023A-B9 | VGB6 | C57BL/6NTac | |
| 15023A-E8 | VGB6 | C57BL/6NTac | |
| 15023A-H6 | VGB6 | C57BL/6NTac | |
