15026 Slc44a1 Status: ES cell colonies screened / QC no positives
| Gene | Slc44a1 |
| Chromosome | 4 |
| Deletion Start | 53,440,687 |
| Deletion End | 53,481,549 |
| Deletion Size | 40,863 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,441,154 | 53,441,173 | CGGGATCAGGTGCTTCACTG |
| TUP | 53,441,182 | 53,441,203 | TGCCCTTCCAGGGCCCATTTCA |
| TUR | 53,441,204 | 53,441,222 | AACGAGCGGGTGGGATCTG |
| TDF | 53,480,974 | 53,480,992 | GGTTCAGGGCTGGGCTTAG |
| TDP | 53,480,993 | 53,481,015 | CTTGGCTGGTAGGTGCTTGCCCA |
| TDR | 53,481,052 | 53,481,071 | GCCATCCATCTGGCTCATTG |
PCR Primers
| Primer | Sequence |
| SU | TTTCCGCTGGGGAGCAGCT |
| SD | GGTCCTTCATTTTCATCTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
