15078 O3far1 Status: ES cell colonies screened / QC positives
| Gene | O3far1 |
| Chromosome | 19 |
| Deletion Start | 38,171,620 |
| Deletion End | 38,188,586 |
| Deletion Size | 16,967 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,171,681 | 38,171,700 | GCACCCACTTCCCTTTCTTC |
| TUP | 38,171,701 | 38,171,722 | TCGGATGTCAAGGGCGACCACC |
| TUR | 38,171,728 | 38,171,746 | TCTCCACGACGCTCAACAC |
| TDF | 38,188,109 | 38,188,127 | CGAAAGCATCGCGGAAGAG |
| TDP | 38,188,131 | 38,188,156 | TACGCTGAGCTTGGCATACTCTGAGA |
| TDR | 38,188,183 | 38,188,200 | GCGGAAGAGTCGGTAGTC |
PCR Primers
| Primer | Sequence |
| SU | TTAAGAGCACTTAGACTGCC |
| SD | AGCCACACTACGATTGAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15078A-A9 | VGB6 | C57BL/6NTac | |
| 15078B-F5 | VGB6 | C57BL/6NTac | |
| 15078C-H7 | VGB6 | C57BL/6NTac | |
