| Gene | Ephb3 |
| Chromosome | 16 |
| Deletion Start | 21,205,173 |
| Deletion End | 21,222,611 |
| Deletion Size | 17,439 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GACCTTTGGAGGGCTTAGGTTTC |
| TUP | | | TGAACCCTCACCAACAGGGATTTACCC |
| TUR | | | TCACTGAGCCCACAGACAAG |
| TDF | | | TGCTGGGTTTGCATCCTTTG |
| TDP | | | CCTGGTGGCCCAGATGACTGCAG |
| TDR | | | TGGGCTCAGCCAGAGTTC |
PCR Primers
| Primer | Sequence |
| SU | AGCAGAAGTGAGTACGTGAG |
| SD | GAGGTACCAGCTGGCTTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15079A-C5 | VGB6 | C57BL/6NTac | |
| 15079A-D4 | VGB6 | C57BL/6NTac | |
| 15079A-G8 | VGB6 | C57BL/6NTac | |
