| Gene | Wnt4 |
| Chromosome | 4 |
| Deletion Start | 137,277,683 |
| Deletion End | 137,296,837 |
| Deletion Size | 19,155 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 137,278,492 | 137,278,513 | AGACTCCTTCATCCCTCCTCAG |
| TUP | 137,278,514 | 137,278,537 | TGGCACTTCCTATGGCCCAGGCTT |
| TUR | 137,278,549 | 137,278,568 | GCTCTGGGCAGGAGAAGTTG |
| TDF | 137,296,512 | 137,296,533 | GCCACATACAGATGAGGACCTG |
| TDP | 137,296,535 | 137,296,557 | TATACCTGGAGCCTAGCCCGGAC |
| TDR | 137,296,564 | 137,296,582 | CACTGCGGATGTCCTGCTC |
PCR Primers
| Primer | Sequence |
| SU | CAGGAGAAGCCCAGTCCC |
| SD | GCCATCTGTTTCCCTGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15088A-F1 | VGB6 | C57BL/6NTac | |
| 15088A-D10 | VGB6 | C57BL/6NTac | |
| 15088A-B2 | VGB6 | C57BL/6NTac | |
