| Gene | Tlr2 |
| Chromosome | 3 |
| Deletion Start | 83,838,771 |
| Deletion End | 83,836,416 |
| Deletion Size | 2,356 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,837,988 | 83,838,006 | CGAGGCTCGGTTCTCACTG |
| TUP | 83,837,953 | 83,837,980 | CTTTAACGAGCTCCTGAAGCTGTTGCGT |
| TUR | 83,837,921 | 83,837,940 | TCGAACTCTACCTCCGACAG |
| TDF | 83,836,966 | 83,836,988 | GTGCCCTTCTCCTGTTGATCTTG |
| TDP | 83,836,944 | 83,836,964 | TCGTAGGTGCCCTGTGCCACC |
| TDR | 83,836,901 | 83,836,919 | AGCCACGCCCACATCATTC |
PCR Primers
| Primer | Sequence |
| SU | ACCAAGGGAGCAATGAGTTG |
| SD | GAAGATCTGGTGGGATGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15097A-C7 | VGB6 | C57BL/6NTac | |
| 15097A-H9 | VGB6 | C57BL/6NTac | |
