15099 Epha7 Status: ES cell colonies screened / QC no positives
| Gene | Epha7 |
| Chromosome | 4 |
| Deletion Start | 28,813,387 |
| Deletion End | 28,821,663 |
| Deletion Size | 8,277 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,814,111 | 28,814,130 | AGAGGCCACCGAGTGAGAAG |
| TUP | 28,814,132 | 28,814,153 | CTCCCGTCACTTGGCCCAGGTC |
| TUR | 28,814,170 | 28,814,189 | AGGCCAGGACTGGCTACTTG |
| TDF | 28,821,147 | 28,821,170 | TCACCTTGAGGGATTGTAATAGTC |
| TDP | 28,821,172 | 28,821,193 | TCCCGGAGTCCTGGGAACTTGC |
| TDR | 28,821,225 | 28,821,242 | TGCCGGTGTCGTAGTCTG |
PCR Primers
| Primer | Sequence |
| SU | TATTGGAGTGTGGAGCAAGC |
| SD | TCTTTCCTGTCAAAAGCTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
