| Gene | Klk5 |
| Chromosome | 7 |
| Deletion Start | 43,842,319 |
| Deletion End | 43,850,917 |
| Deletion Size | 8,599 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,842,796 | 43,842,816 | GGAGGCCAGAATAGGCTTCAG |
| TUP | 43,842,818 | 43,842,841 | TGGACCTGCAGTTGCAGCTGATTG |
| TUR | 43,842,855 | 43,842,875 | TGGGTGTGATTCCCAGTGTTC |
| TDF | 43,850,663 | 43,850,682 | TCGATTGACCTCACCACACC |
| TDP | 43,850,683 | 43,850,704 | CCTGCCTCCTTCTGTGCCTCCA |
| TDR | 43,850,735 | 43,850,754 | CTTGCCATTGCAGACCACAG |
PCR Primers
| Primer | Sequence |
| SU | AACCTGATTTGGCTCTGTCT |
| SD | GCTGCCTGGTACAGGAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15104A-A1 | VGB6 | C57BL/6NTac | |
| 15104A-F3 | VGB6 | C57BL/6NTac | |
| 15104A-F7 | VGB6 | C57BL/6NTac | |
