15112 Thbs1 Status: ES cell colonies screened / QC positives
| Gene | Thbs1 |
| Chromosome | 2 |
| Deletion Start | 117,938,382 |
| Deletion End | 117,950,769 |
| Deletion Size | 12,388 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 117,939,042 | 117,939,061 | CAGGGAAGCAACAAGTGGTG |
| TUP | 117,939,064 | 117,939,087 | AGTGGAGGAAGCTCTCCTGGCCAC |
| TUR | 117,939,116 | 117,939,134 | GAGCCCGGTCCTCTTGAAC |
| TDF | 117,950,417 | 117,950,435 | TTCACGGGATGGCTTACAC |
| TDP | 117,950,438 | 117,950,463 | TGAGATCGGACCTACCGCAGAGTAAC |
| TDR | 117,950,483 | 117,950,504 | TGTGCTTGCAGACATCTGACTC |
PCR Primers
| Primer | Sequence |
| SU | CTGGTTCTCTTCAGCCTTCG |
| SD | AATGCTTCTGTGTGTGCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15112A-A5 | VGB6 | C57BL/6NTac | |
| 15112A-G12 | VGB6 | C57BL/6NTac | |
