| Gene | Sema6c |
| Chromosome | 3 |
| Deletion Start | 95,164,396 |
| Deletion End | 95,173,371 |
| Deletion Size | 8,976 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,164,875 | 95,164,897 | AGACAGACATAAGGAGTGCAAGG |
| TUP | 95,164,899 | 95,164,921 | CTTAGCCACACCAAACCACGGCA |
| TUR | 95,164,927 | 95,164,947 | CGAGCAGATGAGCAGGGTTTC |
| TDF | 95,172,784 | 95,172,802 | AGAGGTGACCACGCTGGAG |
| TDP | 95,172,805 | 95,172,826 | ACTGCTGCGCTACCTGCACGGC |
| TDR | 95,172,838 | 95,172,858 | AGAGGTTCGCTACCCTTCCTG |
PCR Primers
| Primer | Sequence |
| SU | AGAGGAAGAAAGGCTGGGAG |
| SD | AATTGCTCCGGAGAGACTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15114A-H5 | VGB6 | C57BL/6NTac | |
| 15114A-D11 | VGB6 | C57BL/6NTac | |
| 15114A-E6 | VGB6 | C57BL/6NTac | |
| 15114A-E5 | VGB6 | C57BL/6NTac | |
| 15114A-E8 | VGB6 | C57BL/6NTac | |
