15124 Reg4 Status: ES cell colonies screened / QC no positives
| Gene | Reg4 |
| Chromosome | 3 |
| Deletion Start | 98,224,777 |
| Deletion End | 98,236,427 |
| Deletion Size | 11,651 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,225,418 | 98,225,437 | AGCTTGGTCATCTCCCACAG |
| TUP | 98,225,441 | 98,225,464 | CCCACAACCTCACTGGGAGTTGGA |
| TUR | 98,225,498 | 98,225,522 | CCCTCGACAGAACTAATGCTCAAAC |
| TDF | 98,235,922 | 98,235,945 | GCAAGAGCTGATGTGGAAAGATTG |
| TDP | 98,235,953 | 98,235,981 | AGCCATCAGAGACAGCATTTTCTGGCTCA |
| TDR | 98,236,009 | 98,236,031 | TGGGTGGGTCTCGTCTAATAATG |
PCR Primers
| Primer | Sequence |
| SU | TAGGACTCTGGCATTGGTCC |
| SD | GAACATTCCATGGCAAACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
