| Gene | Gpr142 |
| Chromosome | 11 |
| Deletion Start | 114,798,927 |
| Deletion End | 114,806,755 |
| Deletion Size | 7,829 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 114,799,315 | 114,799,338 | GCTGGATGGATGAACTGGTGATAG |
| TUP | 114,799,348 | 114,799,374 | TTGAGGACCAGGAAGATCAAGGCATGT |
| TUR | 114,799,386 | 114,799,408 | ACCTGGCTGTGAAAGATGTTGTC |
| TDF | 114,806,280 | 114,806,302 | CCCTCCTAGCACTATGGACAAAC |
| TDP | 114,806,304 | 114,806,326 | CCTCAAGTGGGCTCACTGCCTCA |
| TDR | 114,806,364 | 114,806,385 | CCGAAGGATGATGGCAGAGTTG |
PCR Primers
| Primer | Sequence |
| SU | GGTTCCAGGCTCAATACACC |
| SD | TCGACAGCTGTTATGCCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15144A-A7 | VGB6 | C57BL/6NTac | |
| 15144A-A11 | VGB6 | C57BL/6NTac | |
| 15144A-C11 | VGB6 | C57BL/6NTac | |
| 15144A-H4 | VGB6 | C57BL/6NTac | |
