15152 Ccr5 Status: ES cell colonies screened / QC no positives
| Gene | Ccr5 |
| Chromosome | 9 |
| Deletion Start | 124,125,462 |
| Deletion End | 124,124,365 |
| Deletion Size | 1,098 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,124,371 | 124,124,390 | CAAGGGTCAGTTCCGACCTA |
| TUP | 124,124,400 | 124,124,428 | CATCGATTATGGTATGTCAGCACCCTGCC |
| TUR | 124,124,435 | 124,124,456 | GCCGCAATTTGTTTCACATTGA |
| TDF | 124,125,299 | 124,125,322 | TCTTCCGAAAACACATGGTCAAAC |
| TDP | 124,125,327 | 124,125,353 | TTGCAAACGGTGTTCAATTTTCCAGCA |
| TDR | 124,125,379 | 124,125,399 | TCCTGTGGATCGGGTATAGAC |
PCR Primers
| Primer | Sequence |
| SU | CCCACTACTCATTCTTTCTGGC |
| SD | GCAAGAAGCGACTTTATGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
