15154 Cacna1i Status: Cancelled
| Gene | Cacna1i |
| Chromosome | 15 |
| Deletion Start | 80,321,316 |
| Deletion End | 80,298,418 |
| Deletion Size | 22,899 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,298,571 | 80,298,588 | GCACCCGTTGCCTTCTTC |
| TUP | 80,298,589 | 80,298,608 | TGCCTGCGCCAGACCACGAG |
| TUR | 80,298,615 | 80,298,639 | ACATACCATCTTGATGCACCAGTTC |
| TDF | 80,320,660 | 80,320,679 | CAGCCTCTGCTCCCTGATAC |
| TDP | 80,320,682 | 80,320,703 | CCTCCCTTGTGGACTCGTGGGC |
| TDR | 80,320,727 | 80,320,745 | TACCTCACACCGTGCCTAC |
PCR Primers
| Primer | Sequence |
| SU | CTATCACATGCCACACTCCC |
| SD | AAAACACTCAGTCTAAGCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
